The effect of heme biosynthesis on the mitochondrial functions in C2C12 cels

Arif Suprihadi; Hung Wei Lai; Hui Sun Tan; Kiwamu Takahashi; Motowo Nakajima; Tohru Tanaka; Shun-ichiro Ogura

doi:10.1117/12.2525559

7 August 2019 The effect of heme biosynthesis on the mitochondrial functions in C2C12 cels

Arif Suprihadi, Hung Wei Lai, Hui Sun Tan, Kiwamu Takahashi, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura

Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 11070A4 (2019) https://doi.org/10.1117/12.2525559
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States

Abstract

Mitochondria play a fundamental role in generating of energy in cells. Electron transfer in electron transport chain (ETC) involved in mitochondria was one of the biological functions of heme. Heme biosynthesizes increased upon the addition of 5-aminolevulinic acid (ALA). Complex I and II protein expression were downregulated, while complex IV and cytochrome c expression were upregulated by the addition of ALA. Administration of ALA significantly increased Complex IV (COX) activity and cellular ATP level. Surprisingly, the mitochondrial content and mitochondrial membrane potential were unchanged. Consistently, the relative mRNA-expression of transcription factors affecting mitochondrial biogenesis was unchanged after the addition of ALA.

Citation Download Citation

Arif Suprihadi, Hung Wei Lai, Hui Sun Tan, Kiwamu Takahashi, Motowo Nakajima, Tohru Tanaka, and Shun-ichiro Ogura "The effect of heme biosynthesis on the mitochondrial functions in C2C12 cels", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 11070A4 (7 August 2019); https://doi.org/10.1117/12.2525559

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available