Detection of trichomonal vaginalis through lensless optofluidic microscopy

Jessica Kun; Marek Smieja; Leyla Soleymani; Qiyin Fang

doi:10.1117/12.2526893

22 July 2019 Detection of trichomonal vaginalis through lensless optofluidic microscopy

Jessica Kun, Marek Smieja, Leyla Soleymani, Qiyin Fang

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110761A (2019) https://doi.org/10.1117/12.2526893
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Trichomoniasis is one of the most common non-viral sexually transmitted infection caused by a parasite called Trichomonas vaginalis. Currently, there is no timely, cost-efficient diagnostic test exist for Trichomoniasis. We present a lensless optofluidic imaging technique for label-free point-of-care detection of motile parasites in bodily fluids. With this setup we are able to achieve a FOV of ~2 mm² at a frame rate of 30 fps and can see ~200 μl of sample in one image. Trichomonas vaginalis is identifiable through simple morphological features. Contrary to common practice of minimizing the distance between the sample and the sensor to maximize the resolution, we demonstrated that a greater distance is more advantageous by using the parasites natural ability to focus light as a contrast mechanism. This technique uses a low-cost high frame rate CMOS detector, which enables high throughput operation with requires minimal sample preparation, making it a promising solution to the rapid diagnosis of Trichomoniasis.

Citation Download Citation

Jessica Kun, Marek Smieja, Leyla Soleymani, and Qiyin Fang "Detection of trichomonal vaginalis through lensless optofluidic microscopy", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761A (22 July 2019); https://doi.org/10.1117/12.2526893

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