Phage susceptibility testing with lensless imaging technique (Conference Presentation)

Prisca Perlemoine; Emmanuel Picard; Emmanuel Hadji; Marc Zelsmann; Alexis Maire; Eric Lacot; Pierre Marcoux

doi:10.1117/12.2543785

10 March 2020 Phage susceptibility testing with lensless imaging technique (Conference Presentation)

Prisca Perlemoine, Emmanuel Picard, Emmanuel Hadji, Marc Zelsmann, Alexis Maire, Eric Lacot, Pierre Marcoux

Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 112230D (2020) https://doi.org/10.1117/12.2543785
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

With the rise of antibiotic resistance, phage therapy is seen as a promising alternative to cure infection to multiresistant bacteria strains. However, phage susceptibility tests currently carried out are time-consuming and are not compliant with the automated environment of hospital laboratories. In this work, we present a method for phage susceptibility testing through optical density measurement with the use of lensless imaging technique. Using a 3.3 cm2 area CANON sensor and a custom test card, we are able to simultaneously monitor the bacterial growth or inhibition of multiple bacterial/phage samples and to provide reliable results in less than 4 hour.

Conference Presentation

Citation Download Citation

Prisca Perlemoine, Emmanuel Picard, Emmanuel Hadji, Marc Zelsmann, Alexis Maire, Eric Lacot, and Pierre Marcoux "Phage susceptibility testing with lensless imaging technique (Conference Presentation)", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230D (10 March 2020); https://doi.org/10.1117/12.2543785

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