Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains

Jie Hui; Pu-Ting Dong; Lijia Liang; Taraknath Mandal; Junjie Li; Erlinda R. Ulloa; Yuewei Zhan; Sebastian Jusuf; Cheng Zong; Mohamed N. Seleem; George Y. Liu; Qiang Cui; Ji-Xin Cheng

doi:10.1117/12.2543045

19 February 2020 Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains

Jie Hui, Pu-Ting Dong, Lijia Liang, Taraknath Mandal, Junjie Li, Erlinda R. Ulloa, Yuewei Zhan, Sebastian Jusuf, Cheng Zong, Mohamed N. Seleem, George Y. Liu, Qiang Cui, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 112230T (2020) https://doi.org/10.1117/12.2543045
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The rapid evolution of antibiotic resistance increasingly challenges the successful treatment of S. aureus infections. Here, we present an unconventional treatment approach by disassembly its membrane microdomains via pulsed laser photolysis of staphyloxanthin. After staphyloxanthin photolysis, membrane permeabilization, fluidification, and membrane protein detachment, were found the underlying mechanisms to malfunction its defense to several major classes of conventional antibiotics. Through resistance selection study, we found pulsed laser treatment completely depleted staphyloxanthin virulence. More importantly, laser treatment further inhibited development of resistance for several major classes of conventional antibiotics including fluoroquinolones, tetracyclines, aminoglycosides, and oxazolidinones. Collectively, this work highlights a novel platform to revive conventional antibiotics to treat S. aureus infections.

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Citation Download Citation

Jie Hui, Pu-Ting Dong, Lijia Liang, Taraknath Mandal, Junjie Li, Erlinda R. Ulloa, Yuewei Zhan, Sebastian Jusuf, Cheng Zong, Mohamed N. Seleem, George Y. Liu, Qiang Cui, and Ji-Xin Cheng "Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230T (19 February 2020); https://doi.org/10.1117/12.2543045

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