Two-photon Bessel beam scanning microscope for neural activities (Conference Presentation)

Dongli Xu; Leilei Peng; Jun Ding

doi:10.1117/12.2546526

9 March 2020 Two-photon Bessel beam scanning microscope for neural activities (Conference Presentation)

Dongli Xu, Leilei Peng, Jun Ding

Proceedings Volume 11226, Neural Imaging and Sensing 2020; 1122603 (2020) https://doi.org/10.1117/12.2546526
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Neuroscience research is propelled by advance imaging tools, which ideally could provide high spatial and temporal resolution imaging. However, available fast 3-dimensional optical sectioning approaches applicable to deep tissue and large scale in-vivo imaging are still limited. We develop a novel two-photon Bessel beam scanning light sheet microscope, which has two distinct imaging modes: 1) The light-sheet imaging mode enables sub-micron 3D imaging over hundreds of microns field of view, which is well adapted to the neural tissue with complex structure; 2) The projection imaging mode aims to capture fast neural activities at up to 100 volumes per second. Imaging results of various neural specimens will be presented.

Conference Presentation

Citation Download Citation

Dongli Xu, Leilei Peng, and Jun Ding "Two-photon Bessel beam scanning microscope for neural activities (Conference Presentation)", Proc. SPIE 11226, Neural Imaging and Sensing 2020, 1122603 (9 March 2020); https://doi.org/10.1117/12.2546526

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available