Effect of blue light irradiation on human skin by in vivo confocal Raman spectroscopy

Larissa K. Z. R. Bello; Priscila P. Fávero; Gustavo C. da Silva; Airton A. Martin

doi:10.1117/12.2552836

21 February 2020 Effect of blue light irradiation on human skin by in vivo confocal Raman spectroscopy

Larissa K. Z. R. Bello, Priscila P. Fávero, Gustavo C. da Silva, Airton A. Martin

Proceedings Volume 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry; 1123612 (2020) https://doi.org/10.1117/12.2552836
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Visible component of sunlight has a physiologically significant effect on human skin and long-term exposure to concentrated blue light energy (sunlight, laptop, cell phones) could produce oxidative stress leading to the premature skin aging. In this work, in vivo confocal Raman spectroscopy was used to characterize biochemical changes in human skin after been irradiated with different doses of blue light. After ethical committee approval, volunteers’ phototype I and II (Fitzpatrick classification) have been selected. The River Diagnosis confocal Raman spectrometer was used, before (T0) and after 15, 30 and 60 minutes of blue light irradiation (LED 450 nm) with doses of 100 J/cm² . It was possible to evaluate the biochemical skin damage caused in the stratum corneum and in the viable epidermis.

Citation Download Citation

Larissa K. Z. R. Bello, Priscila P. Fávero, Gustavo C. da Silva, and Airton A. Martin "Effect of blue light irradiation on human skin by in vivo confocal Raman spectroscopy", Proc. SPIE 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry, 1123612 (21 February 2020); https://doi.org/10.1117/12.2552836

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KEYWORDS

Skin

Raman spectroscopy

Confocal microscopy

In vivo imaging

Visible radiation

Analytical research

Light emitting diodes

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