Mapping the phonon dispersion in biological matter using angle-resolved Brillouin light scattering microspectroscopy

Kareem Elsayad

doi:10.1117/12.2550449

13 February 2020 Mapping the phonon dispersion in biological matter using angle-resolved Brillouin light scattering microspectroscopy

Kareem Elsayad

Author Affiliations +

Proceedings Volume 11242, Optical Elastography and Tissue Biomechanics VII; 112420F (2020) https://doi.org/10.1117/12.2550449
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Biological matter is usually structurally anisotropic. As such the speed of acoustic phonons, which can be probed using Brillouin spectroscopy, depends on the angular-direction. Using a novel spectrometer-setup, which simultaneously probes the acoustic-velocity from different azimuthal angles, we quantify this anisotropy in different biological structures. By point-scanning the sample we render spatial-maps of parts of the phonon dispersion relation and find different regions in live cells have distinct anisotropic properties in their phonon velocity, with variations related to cell-health and phase. I will discuss the physical/biological interpretation of our results, their relevance for understanding/modelling cellular mechanics, and potential diagnostic applications.

Conference Presentation

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Kareem Elsayad "Mapping the phonon dispersion in biological matter using angle-resolved Brillouin light scattering microspectroscopy", Proc. SPIE 11242, Optical Elastography and Tissue Biomechanics VII, 112420F (13 February 2020); https://doi.org/10.1117/12.2550449

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