Time-gated fluorescence imaging and sensing using long lifetime near infrared quantum dots (Conference Presentation)

Thomas Pons; Sophie Bouccara; Manon Debayle; Vincent Loriette; Nicolas Lequeux; Alexandra Fragola

doi:10.1117/12.2545627

9 March 2020 Time-gated fluorescence imaging and sensing using long lifetime near infrared quantum dots (Conference Presentation)

Thomas Pons, Sophie Bouccara, Manon Debayle, Vincent Loriette, Nicolas Lequeux, Alexandra Fragola

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430W (2020) https://doi.org/10.1117/12.2545627
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Time-gated fluorescence imaging of near-infrared emitting ZnCuInSe/ZnS quantum dots (QDs) with fluorescence lifetimes in the range of 150−300 ns enables the efficient rejection of fast autofluorescence photons and the selection of QD fluorescence photons. This leads to complete elimination of autofluorescence background and a significant increase of imaging sensitivity. We demonstrate efficient detection and imaging of individual QD-labeled lymphoma cells circulating at mm/s velocities in blood vessels. In a second application, these QDs were used as a sensing platform to detect enzymatic activity using a ratiometric time-gated sensing scheme.

Conference Presentation

Citation Download Citation

Thomas Pons, Sophie Bouccara, Manon Debayle, Vincent Loriette, Nicolas Lequeux, and Alexandra Fragola "Time-gated fluorescence imaging and sensing using long lifetime near infrared quantum dots (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430W (9 March 2020); https://doi.org/10.1117/12.2545627

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KEYWORDS

Luminescence

Near infrared

Quantum dots

In vivo imaging

Absorption

Auto-fluorescence imaging

Diffusion

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