Multiscale SHG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Conference Presentation)

Paul J. Campagnola; Darian S. James; Zachary Alden; Hsin-Yu Chang; Mark Jones; Nathan Sandbo

doi:10.1117/12.2544902

9 March 2020 Multiscale SHG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Conference Presentation)

Paul J. Campagnola, Darian S. James, Zachary Alden, Hsin-Yu Chang, Mark Jones, Nathan Sandbo

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124412 (2020) https://doi.org/10.1117/12.2544902
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We use SHG microscopy to study the collagen alterations in idiopathic pulmonary fibrosis (IPF) in ex vivo human tissues and in vitro spheroid models. We found the collagen fibril and macromolecular structures are altered in IPF, consistent with decreased organization. Further, machine learning based texture analysis successfully classified the fiber morphology at near 100% accuracy. To gain insight into the underlying mechanisms of these alterations, we grew IPF based spheroids under different crosslinking conditions (increased or inhibited). SHG polarization measurements (linear and SHG-CD) indicated that increased crosslinking altered the collagen helical structure similar to those of the human tissues.

Conference Presentation

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Paul J. Campagnola, Darian S. James, Zachary Alden, Hsin-Yu Chang, Mark Jones, and Nathan Sandbo "Multiscale SHG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124412 (9 March 2020); https://doi.org/10.1117/12.2544902

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KEYWORDS

Collagen

Second-harmonic generation

Pulmonary fibrosis

Tissues

Dichroic materials

Image classification

In vitro testing

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