Towards intracellular phase transitions in ALS disease by noncontact Brillouin microscopy (Conference Presentation)

Giuseppe Antonacci; Valeria de Turris; Alessandro Rosa; Giancarlo Ruocco

doi:10.1117/12.2545642

10 March 2020 Towards intracellular phase transitions in ALS disease by noncontact Brillouin microscopy (Conference Presentation)

Giuseppe Antonacci, Valeria de Turris, Alessandro Rosa, Giancarlo Ruocco

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 1125108 (2020) https://doi.org/10.1117/12.2545642
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Altered biomechanics and phase transitions are implicated as key photogenic triggers in neurodegenerative diseases. However, standard methods to measure them require invasive contact with the sample or provide low spatial resolution. Here, we demonstrate Brillouin microscopy as a potential tool to investigate liquid-to-solid phase transitions in intracellular compartments in response to expression of ALS-linked proteins. In particular, we show how intracellular stress granules exhibit altered biomechanics in response to recruitment of RNA-binding proteins, such as ELAVL4 and FUS. Results pave the way to a better understanding of the dynamics that lead to formation of solid aggregates during neurodegeneration.

Conference Presentation

Citation Download Citation

Giuseppe Antonacci, Valeria de Turris, Alessandro Rosa, and Giancarlo Ruocco "Towards intracellular phase transitions in ALS disease by noncontact Brillouin microscopy (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 1125108 (10 March 2020); https://doi.org/10.1117/12.2545642

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