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The recent development and integration of multiple non-linear microscopy techniques in a single instrument has provided new opportunities for integrating morphological and functional information and for correlating the observed molecular and cellular changes with disease behaviour. In particular, multimodal non-linear/linear imaging is able to perform a morpho-chemical quantitative analysis in tumour cells and tissue specimens, providing a high-resolution label-free alternative to both histological and immune-histochemical examination of tissues.
In this talk, a brief overview on the non linear and linear laser brain imaging techniques will be displayed for label free detection.
Morpho-chemical characterization of tissue will be displayed as an interesting tool for early diagnosis of pathologies: different kind of approaches will be shown for in vivo imaging assisted surgery operation or as tools to support anatomo-pathologists decision.
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