Metal enhanced fluorescence using nanostructures on silver formed with Ti: Saphire femtosecond pulsed laser

Vicente S. Mattos; Fernanda R. Paolillo; Daniel Cavallini; Marcelo A. Pereira-da-Silva; Marco A. A. de Souza; Fatima M. M. Yasuoka; Francisco E. G. Guimarães; Jarbas C. de Castro Neto

doi:10.1117/12.2545963

2 March 2020 Metal enhanced fluorescence using nanostructures on silver formed with Ti: Saphire femtosecond pulsed laser

Vicente S. Mattos, Fernanda R. Paolillo, Daniel Cavallini, Marcelo A. Pereira-da-Silva, Marco A. A. de Souza, Fatima M. M. Yasuoka, Francisco E. G. Guimarães, Jarbas C. de Castro Neto

Author Affiliations +

Proceedings Volume 11268, Laser-based Micro- and Nanoprocessing XIV; 1126822 (2020) https://doi.org/10.1117/12.2545963
Event: SPIE LASE, 2020, San Francisco, California, United States

Abstract

Metal enhanced fluorescence is a phenomenon that occurs when a fluorophore is positioned near a conductive structure. Structures can be formed by the interaction of high-energy laser with a sample, on which the femtosecond laser pulses interact with the surface without heat effects. This work aims to study nanostructure formation in polished bulk silver, in order to amplify signals of fluorescence. A Titanium-sapphire femtosecond laser was used to mark silver surfaces. SEM images shows nanostructures formed in chaotic agglomerate of nanospheres with size of 50-800nm. Using Protoporphyrin-IX, the fluorescence amplification was around 300 times compared to a surface without the nanostructures.

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Vicente S. Mattos, Fernanda R. Paolillo, Daniel Cavallini, Marcelo A. Pereira-da-Silva, Marco A. A. de Souza, Fatima M. M. Yasuoka, Francisco E. G. Guimarães, and Jarbas C. de Castro Neto "Metal enhanced fluorescence using nanostructures on silver formed with Ti: Saphire femtosecond pulsed laser", Proc. SPIE 11268, Laser-based Micro- and Nanoprocessing XIV, 1126822 (2 March 2020); https://doi.org/10.1117/12.2545963

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