Widely-tunable femtosecond source tunable between 770-1180 nm for two-photon fluorescence microscopy

Lu-Ting Chou; Dong-Lin Zhong; Yu-Cheng Liu; Jye-Chang Lee; Yi-Syuan Chen; Shih-Hsuan Chia

doi:10.1117/12.2545562

2 March 2020 Widely-tunable femtosecond source tunable between 770-1180 nm for two-photon fluorescence microscopy

Lu-Ting Chou, Dong-Lin Zhong, Yu-Cheng Liu, Jye-Chang Lee, Yi-Syuan Chen, Shih-Hsuan Chia

Proceedings Volume 11270, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XX; 1127015 (2020) https://doi.org/10.1117/12.2545562
Event: SPIE LASE, 2020, San Francisco, California, United States

Abstract

We have demonstrated a widely-tunable femtosecond fiber source between 770-1180 nm enabled by self-phase modulation, and the wide spectral coverage is suitable for most of two-photon fluorescence microscopy applications. Based on femtosecond Yb:fiber laser, we also compared spectral broadening in different dispersion regimes using different photonic crystal fibers. We managed to maximize the self-phase modulated feature from the broadened spectra while avoiding unwanted nonlinear temporal trapping. The optimization of fiber selection and laser input conditions led to a wide tunability down to below 800nm region, which is the most commonly used two-photon excitation wavelength for many intrinsic fluorescent labels in biological tissues. We believe this fiber-based femtosecond source can be a relatively cost-effective and robust solution for most of two-photon fluorescence microscopy applications.

Conference Presentation

Citation Download Citation

Lu-Ting Chou, Dong-Lin Zhong, Yu-Cheng Liu, Jye-Chang Lee, Yi-Syuan Chen, and Shih-Hsuan Chia "Widely-tunable femtosecond source tunable between 770-1180 nm for two-photon fluorescence microscopy", Proc. SPIE 11270, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XX, 1127015 (2 March 2020); https://doi.org/10.1117/12.2545562

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