Open-source 3D-printed digital inline holographic microscope for low-cost cellular imaging

Stephan Amann; Max von Witzleben; Stefan Breuer

doi:10.1117/12.2547120

21 February 2020 Open-source 3D-printed digital inline holographic microscope for low-cost cellular imaging

Stephan Amann, Max von Witzleben, Stefan Breuer

Proceedings Volume 11306, Practical Holography XXXIV: Displays, Materials, and Applications; 113060B (2020) https://doi.org/10.1117/12.2547120
Event: SPIE OPTO, 2020, San Francisco, California, United States

Abstract

Digital inline holographic microscopy is a promising cellular object imaging modality. We report on two cost-efficient lens-less experimental set-ups comprising of a standard LED or a semiconductor laser light source with a Raspberry Pi Camera for image acquisition. The microscope parts are 3D-printed yielding a highly compact and portable microimaging solution. Tobacco cells, human red blood cells and polystyrene microspheres are successfully imaged by an open-source reconstruction software. The developed microscopes are cost-efficient (<$200) and yield spatial resolutions of 3.91 μm respectively 1.55 μm. They constitute a flexible tool for science and student and early researcher education that can be tailored to the researchers demand. All employed code is open-source accessible aiming at triggering further developments and sharing between research laboratories, diagnostic labs and science education.

Conference Presentation

Citation Download Citation

Stephan Amann, Max von Witzleben, and Stefan Breuer "Open-source 3D-printed digital inline holographic microscope for low-cost cellular imaging", Proc. SPIE 11306, Practical Holography XXXIV: Displays, Materials, and Applications, 113060B (21 February 2020); https://doi.org/10.1117/12.2547120

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