Ion And Optical Microscopic Images Superimposition And Correlation

J. C. Olivo; E. Kahn; S. Halpern; P. Fragu; R. Di Paola

doi:10.1117/12.961644

11 October 1989 Ion And Optical Microscopic Images Superimposition And Correlation

J. C. Olivo, E. Kahn, S. Halpern, P. Fragu, R. Di Paola

Proceedings Volume 1135, Image Processing III; (1989) https://doi.org/10.1117/12.961644
Event: 1989 International Congress on Optical Science and Engineering, 1989, Paris, France

Abstract

To make biological interpretation of Analytical Ion Microscopy (AIM) images possible, computerised superimposition of ion and optical images has become necessary. By these means, appropriate identification of morphological structures is achievable and functional activity of a tissue can be assessed. Although a sequence of ion images of different mass numbers refers to the same field, slight topographic differences can be observed. They often result in image-to-image misregistrations and improper localisation of chemical distributions with respect to other ones. A method is proposed to register ionic images in respect to the optical image of the serial cross section of the same field.

Citation Download Citation

J. C. Olivo, E. Kahn, S. Halpern, P. Fragu, and R. Di Paola "Ion And Optical Microscopic Images Superimposition And Correlation", Proc. SPIE 1135, Image Processing III, (11 October 1989); https://doi.org/10.1117/12.961644

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