SERS signal of serum proteins purified with cellulose acetate membrane

Shuzhen Tang; Tingting Lin; Yun Yu; Jianshu Xu; Mengmeng Zheng; Yimei Huang; Juqiang Lin

doi:10.1117/12.2574362

10 October 2020 SERS signal of serum proteins purified with cellulose acetate membrane

Shuzhen Tang, Tingting Lin, Yun Yu, Jianshu Xu, Mengmeng Zheng, Yimei Huang, Juqiang Lin

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115532D (2020) https://doi.org/10.1117/12.2574362
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

Serum proteins contain many biomarkers of diseases, such as cancer. It would be important to purify the serum proteins for disease diagnosis. In this paper, cellulose acetate (CA) membrane was employed to purify serum proteins from human serum while removing other serum constituents and exogenous substances. The purified serum proteins were mixed with silver nanoparticles for SERS measurement. A total of 42 SERS spectra were recorded from purified serum proteins obtained from 20 liver cancer patients and 22 healthy volunteers. Subtle but discernible spectral changes of the two groups could be observed in the SERS spectra. Principal components analysis (PCA) and linear discriminate analysis (LDA) algorithm were introduced to analyze the difference between the two groups. Additionally, this method is nondestructive, fast and easy to operate, which is greatly important for clinical serum sample detection. These results indicated that SERS signal of serum proteins purified with CA membrane has a good prospect in liver cancer screening.

Citation Download Citation

Shuzhen Tang, Tingting Lin, Yun Yu, Jianshu Xu, Mengmeng Zheng, Yimei Huang, and Juqiang Lin "SERS signal of serum proteins purified with cellulose acetate membrane", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115532D (10 October 2020); https://doi.org/10.1117/12.2574362

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available