The diagnostic potential of advanced glycation end products (AGE) estimation by its autofluorescence is restricted by not sufficient specificity and high level of variability even in case of the same individual. One of the reasons for this restriction is melanin influence on the signal measured by diagnostic fluorescence meter, as melanin is a strong endogenous chromophore. In addition, the AGE autofluorescence is excited by ultraviolet or violet radiation, the penetration depth of which into the skin is small. In order to decrease such influence, the green LED with a peak wavelength of 530 nm was implemented. The ratio of the fluorescent radiation intensity to the product of the two intensities of the elastically scattered skin radiation was used. One of them was scattering of ultraviolet LED light with a peak wavelength of 365 nm that excited autofluorescence, the other was green LED light. This approach results in two times lower melanin influence on AGE autofluorescence.
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