Characterization of RBC flux and velocity measurements in OCT with concurrent two-photon microscopy

Paul J. Marchand; Xuecong Lu; Cong Zhang; Frédéric Lesage

doi:10.1117/12.2583157

5 March 2021 Characterization of RBC flux and velocity measurements in OCT with concurrent two-photon microscopy

Paul J. Marchand, Xuecong Lu, Cong Zhang, Frédéric Lesage

Proceedings Volume 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV; 1163017 (2021) https://doi.org/10.1117/12.2583157
Event: SPIE BiOS, 2021, Online Only

Abstract

As red blood cells flowing in capillaries pass through the focal volume of an optical coherence tomography (OCT) system, they will induce a transient change in back-scattering, leading to peaks in the intensity of the OCT signal. In recent years, an OCT method has been devised exploiting these intensity fluctuations to estimate the hemodynamic parameters, such as RBC speed and flux. In this work, using concurrent OCT and two-photon microscopy, we assess the accuracy of the technique, highlighting that single red blood cells can indeed be detected in OCT, in specific hemodynamic and experimental settings.

Conference Presentation

Citation Download Citation

Paul J. Marchand, Xuecong Lu, Cong Zhang, and Frédéric Lesage "Characterization of RBC flux and velocity measurements in OCT with concurrent two-photon microscopy", Proc. SPIE 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV, 1163017 (5 March 2021); https://doi.org/10.1117/12.2583157

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