Characterization of cellular response to extracellular vesicles using two-photon FLIM of NAD(P)H

Janet E. Sorrells; Elisabeth M. Martin; Edita Aksamitiene; Rishyashring R. Iyer; Lingxiao Yang; Marina Marjanovic; Stephen A. Boppart

doi:10.1117/12.2577668

5 March 2021 Characterization of cellular response to extracellular vesicles using two-photon FLIM of NAD(P)H

Janet E. Sorrells, Elisabeth M. Martin, Edita Aksamitiene, Rishyashring R. Iyer, Lingxiao Yang, Marina Marjanovic, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 116470A (2021) https://doi.org/10.1117/12.2577668
Event: SPIE BiOS, 2021, Online Only

Abstract

Extracellular vesicles (EVs) are plasma-membrane formed particles released by cells, and range in diameter from 50 to 2000 nm. Interest in EVs is growing, and recent work has aimed to employ nonlinear optical microscopy techniques to better characterize the size, function, and biochemical makeup of EVs. Previous studies have shown that EVs can modulate gene expression and metabolism of cells that uptake them. Here, we use fluorescence lifetime imaging microscopy (FLIM) of reduced nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H) to monitor the metabolic response of macrophages and other cells to native and foreign EVs and other known cellular activators.

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Janet E. Sorrells, Elisabeth M. Martin, Edita Aksamitiene, Rishyashring R. Iyer, Lingxiao Yang, Marina Marjanovic, and Stephen A. Boppart "Characterization of cellular response to extracellular vesicles using two-photon FLIM of NAD(P)H", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 116470A (5 March 2021); https://doi.org/10.1117/12.2577668

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