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5 March 2021 Getting more from TCSPC FLIM data: SPCImage NG next generation data analysis software
Wolfgang Becker, Vladislav Shcheslavskiy, Axel Bergmann
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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164809 (2021) https://doi.org/10.1117/12.2578326
Event: SPIE BiOS, 2021, Online Only
Abstract
TCSPC FLIM is the most direct way to obtain molecular information from live cells and tissues. Data analysis in molecular FLIM applications is usually complicated by the need of multi-exponential decay analysis in presence of low photon budget. Other issues are uncertainty in the instrument response function (IRF) and extremely long data processing times. bh's new generation SPCImage NG software addresses these problems by a combination of time-domain and frequency-domain analysis, image segmentation, MLE fitting, GPU processing, and IRF modelling. We demonstrate the performance of SPCImage NG for a number of advanced FLIM applications.
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Wolfgang Becker, Vladislav Shcheslavskiy, and Axel Bergmann "Getting more from TCSPC FLIM data: SPCImage NG next generation data analysis software", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164809 (5 March 2021); https://doi.org/10.1117/12.2578326
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KEYWORDS
Fluorescence lifetime imaging
Data analysis
Data processing
Graphics processing units
Image analysis
Image segmentation
Modeling
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