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5 March 2021 Single-shot high-contrast multiplane imaging with a z-splitter prism
Sheng Xiao, Howard Gritton, Hua-an Tseng, Dana Zemel, Xue Han, Jerome Mertz
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Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164906 (2021) https://doi.org/10.1117/12.2581250
Event: SPIE BiOS, 2021, Online Only
Abstract
We present a single-shot multiplane widefield imaging strategy using a z-splitter prism, which can be assembled from off-the-self components and only requires a single camera. We further introduce a novel extended-volume 3D deconvolution strategy to suppress far-out-of-focus fluorescence background to significantly improve the contrast of our recorded images, conferring to our system a capacity for quasi optical sectioning. By swapping in different z-splitter configurations, we can prioritize high speed or large 3D field-of-view imaging depending on the application of interest. Moreover, our system can be readily applied to a variety of imaging modalities in addition to fluorescence, such as phase-contrast and darkfield imaging, making it a versatile tool for a wide range of biological or biomedical imaging applications.
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Sheng Xiao, Howard Gritton, Hua-an Tseng, Dana Zemel, Xue Han, and Jerome Mertz "Single-shot high-contrast multiplane imaging with a z-splitter prism", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164906 (5 March 2021); https://doi.org/10.1117/12.2581250
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KEYWORDS
Prisms
Cameras
Deconvolution
3D image processing
Microscopy
Microscopes
Neuroimaging
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