Snapshot 3D imaging with a gigapixel-scale multi-aperture microscope

Kevin C. Zhou; Colin Cooke; Jaehee Park; Pavan Konda; Mark Harfouche; Roarke Horstmeyer

doi:10.1117/12.2576903

5 March 2021 Snapshot 3D imaging with a gigapixel-scale multi-aperture microscope

Kevin C. Zhou, Colin Cooke, Jaehee Park, Pavan Konda, Mark Harfouche, Roarke Horstmeyer

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490C (2021) https://doi.org/10.1117/12.2576903
Event: SPIE BiOS, 2021, Online Only

Abstract

We present a gigapixel-scale multi-aperture microscope capable of measuring a sample’s 3D height profile over multi-centimeter-scale fields of view with a series of single synchronized camera snapshots. Exploiting the overlap redundancy in our multi-aperture camera array microscope, we developed a novel, end-to-end photogrammetric reconstruction algorithm that simultaneously calibrates the cameras’ 3D positions and poses, stitches the acquired images, and generates a coregistered, pixel-wise 3D height map of the sample. Our work opens the door to video-rate 3D monitoring of dynamic scenes at micrometer-scale resolutions and centimeter-scale fields of view.

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Citation Download Citation

Kevin C. Zhou, Colin Cooke, Jaehee Park, Pavan Konda, Mark Harfouche, and Roarke Horstmeyer "Snapshot 3D imaging with a gigapixel-scale multi-aperture microscope", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490C (5 March 2021); https://doi.org/10.1117/12.2576903

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KEYWORDS

Microscopes

Cameras

Stereoscopy

3D metrology

3D image processing

Temporal resolution

Imaging systems

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