Solid immersion meniscus lens (SIMlens) enables multi-resolution open-top light-sheet microscopy

Lindsey Barner; Adam K. Glaser; Hongyi Huang; Jonathan T. C. Liu

doi:10.1117/12.2576560

5 March 2021 Solid immersion meniscus lens (SIMlens) enables multi-resolution open-top light-sheet microscopy

Lindsey Barner, Adam K. Glaser, Hongyi Huang, Jonathan T. C. Liu

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490K (2021) https://doi.org/10.1117/12.2576560
Event: SPIE BiOS, 2021, Online Only

Abstract

Open-top light-sheet (OTLS) microscopy has recently been developed as a high-throughput, easy-to-use 3D microscopy technique for large specimens [1-2]. The oblique angle of the optical beams relative to the sample plate introduces challenges however, and previous solutions (such as a solid immersion lens) have been limiting [1]. Therefore we have developed a solid immersion meniscus lens (SIMlens), which enables optical beams to transition from air to a higher-index immersion medium without introducing aberrations [3]. A SIMlens is compatible with a turret of air objectives, enabling efficient multi-resolution workflows [4]. We present the first multi-resolution OTLS microscope, enabled by a SIMlens.

Conference Presentation

Citation Download Citation

Lindsey Barner, Adam K. Glaser, Hongyi Huang, and Jonathan T. C. Liu "Solid immersion meniscus lens (SIMlens) enables multi-resolution open-top light-sheet microscopy", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490K (5 March 2021); https://doi.org/10.1117/12.2576560

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