Real-time phasor analysis of fast FLIM for visualizing metabolic dynamics

Janet E. Sorrells; Rishyashring R. Iyer; Lingxiao Yang; Andrew J. Bower; Marina Marjanovic; Stephen A. Boppart

doi:10.1117/12.2576001

5 March 2021 Real-time phasor analysis of fast FLIM for visualizing metabolic dynamics

Janet E. Sorrells, Rishyashring R. Iyer, Lingxiao Yang, Andrew J. Bower, Marina Marjanovic, Stephen A. Boppart

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 1165503 (2021) https://doi.org/10.1117/12.2576001
Event: SPIE BiOS, 2021, Online Only

Abstract

Fluorescence lifetime imaging microscopy (FLIM) of reduced nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide phosphate, together termed NAD(P)H, is often used to analyze and characterize the metabolic state of biological samples. The use of photon counting in FLIM of NAD(P)H often leads to long accumulation times which average out sub-second dynamics. This challenge is overcome with the presented two-photon fast FLIM system that uses an analog detection system and fast digitizer, enabling imaging up to 20 frames per second. Furthermore, GPU processing is used for real-time phasor analysis and visualization of collected data.

Conference Presentation

Citation Download Citation

Janet E. Sorrells, Rishyashring R. Iyer, Lingxiao Yang, Andrew J. Bower, Marina Marjanovic, and Stephen A. Boppart "Real-time phasor analysis of fast FLIM for visualizing metabolic dynamics", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 1165503 (5 March 2021); https://doi.org/10.1117/12.2576001

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