Optofluidic flow meter for sub–nanoliter per minute flow measurements

Jalal Sadeghi; Paul N. Patrone; Anthony J. Kearsley; Gregory A. Cooksey

doi:10.1117/12.2592594

20 June 2021 Optofluidic flow meter for sub–nanoliter per minute flow measurements

Jalal Sadeghi, Paul N. Patrone, Anthony J. Kearsley, Gregory A. Cooksey

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117860D (2021) https://doi.org/10.1117/12.2592594
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

Performance improvements in microfluidic systems depend on accurate measurement and control of fluids on the micro- and nanoscale, and new applications are continuously moving the needle to lower volumetric flow rates. This work focuses on improving an optofluidic system for the measurement and calibration of microflows to the sub-nanoliter per minute range. The experimental measurements rely on an optofluidic system that delivers excitation light and records fluorescence in a precise interrogation region of a microfluidic channel. Using a scaling relationship between flow rate and the fluorescence emission after photobleaching, the system enables real-time determination of flow rates. Here we demonstrate improved calibration of a flow controller to 1 % uncertainty and improved resolution of the optofluidic flow meter to less than 1 nL/min using molecules with lower diffusion coefficients.

Conference Presentation

Citation Download Citation

Jalal Sadeghi, Paul N. Patrone, Anthony J. Kearsley, and Gregory A. Cooksey "Optofluidic flow meter for sub–nanoliter per minute flow measurements", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117860D (20 June 2021); https://doi.org/10.1117/12.2592594

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