Determining trap compliances, microsphere size variations, and response linearities in single DNA molecule elasticity measurements with optical tweezers

Mounir Fizari; Youbin Mo; Kristina Koharchik; Douglas E. Smith

doi:10.1117/12.2595172

3 August 2021 Determining trap compliances, microsphere size variations, and response linearities in single DNA molecule elasticity measurements with optical tweezers

Mounir Fizari, Youbin Mo, Kristina Koharchik, Douglas E. Smith

Author Affiliations +

Proceedings Volume 11798, Optical Trapping and Optical Micromanipulation XVIII; 117982F (2021) https://doi.org/10.1117/12.2595172
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Conference Poster

Abstract

Optical tweezers are a powerful tool in biophysics due to the ability to manipulate single biomolecules [1,2]. For dual-trap tweezers with a steerable beam and force detection via back focal plane interferometry and a position sensitive detector [3,4], calibration can be done by stretching single DNA molecules [5,6]. Typically, DNA stretching provides accurate values for 𝛼, the force scale factor that converts from bead displacement from the trap to force, and 𝛽, the conversion between rotation of the piezoelectric controlled mirror and the linear displacement of the movable trap. Determining trap compliance using this method relies on nonlinear fitting that may be sensitive to noise and drift

Citation Download Citation

Mounir Fizari, Youbin Mo, Kristina Koharchik, and Douglas E. Smith "Determining trap compliances, microsphere size variations, and response linearities in single DNA molecule elasticity measurements with optical tweezers", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117982F (3 August 2021); https://doi.org/10.1117/12.2595172

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