Multifunctional and multimodal Fourier domain mode-locked laser-based adaptive optics system for ultrahigh speed retinal cellular imaging

Zhuolin Liu; Furu Zhang; Kelvy Zucca; Daniel X. Hammer

doi:10.1117/12.2608784

4 March 2022 Multifunctional and multimodal Fourier domain mode-locked laser-based adaptive optics system for ultrahigh speed retinal cellular imaging

Zhuolin Liu, Furu Zhang, Kelvy Zucca, Daniel X. Hammer

Author Affiliations +

Proceedings Volume 11941, Ophthalmic Technologies XXXII; 1194105 (2022) https://doi.org/10.1117/12.2608784
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Adaptive optics (AO) has enabled microscopic views of retinal neurons and assessment of their function in living eyes when combined with different imaging modalities, such as scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT). Here, we present a novel design of a multimodal AO imaging system, based on Fourier domain mode-locked (FDML) laser technology. The design allows simultaneous and registered collection of AO-SLO images and AO-OCT volumes at near video rates (13.4 Hz). In addition to the multimodal optical design, the system also features an additional stimulus port and software algorithms to provide multiple functional modes with which to investigate living human retinal cells. The optical system was designed in Zemax with spherical mirrors placed in an out-of-plane configuration to reduce system astigmatism. The system was found to achieve diffraction limited image quality across a 4.5° × 4.5° scanning field. The measured AO-OCT system axial resolution is 8.7 μm in the eye, sensitivity was measured at 88 dB with ~7 dB roll-off over the first ~2 mm. The multimodal system performance was demonstrated by imaging various retinal cells and vessels with co-registered AO-OCT and AO-SLO images. The multifunctional feature was demonstrated by measuring the light-induced phase change of the cone outer segment. The methods will enable development of more sensitive AO-based cellular biomarkers for improved retinal disease diagnosis and treatment.

Conference Presentation

Citation Download Citation

Zhuolin Liu, Furu Zhang, Kelvy Zucca, and Daniel X. Hammer "Multifunctional and multimodal Fourier domain mode-locked laser-based adaptive optics system for ultrahigh speed retinal cellular imaging", Proc. SPIE 11941, Ophthalmic Technologies XXXII, 1194105 (4 March 2022); https://doi.org/10.1117/12.2608784

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