Combining metal induced energy transfer and atomic force microscopy to probe the mechanoresponse of a focal adhesion

Fabian Port; Carolin Grandy; Jonas Pfeil; Kay-Eberhard Gottschalk

doi:10.1117/12.2608108

2 March 2022 Combining metal induced energy transfer and atomic force microscopy to probe the mechanoresponse of a focal adhesion

Fabian Port, Carolin Grandy, Jonas Pfeil, Kay-Eberhard Gottschalk

Author Affiliations +

Proceedings Volume 11967, Single Molecule Spectroscopy and Superresolution Imaging XV; 119670A (2022) https://doi.org/10.1117/12.2608108
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Focal adhesions function are cellular anchoring points to the extracellular matrix and enable cells to sense and exert forces on their environment . They are complex structures consisting of a multitude of different proteins. Despite the important role of the focal adhesion complex in cellular adhesion, its structure and mechanoresponse remain difficult to resolve. Knowing the exact position of the proteins in the focal adhesion complex under strain is necessary to understand their working principle. For a detailed analysis of the focal adhesion architecture coupled with force response, we require a method to measure small distances with super resolution precision while manipulating force acting on the cell. To meet this challenge, we couple life-cell atomic force spectroscopy with Metal Induced Energy Transfer (MIET) to resolve forces with pN and with nanometer accuracy. Here, we show an initial analysis of how forces are transduced from the extracellular space to the actin cytoskeleton.

Conference Presentation

Citation Download Citation

Fabian Port, Carolin Grandy, Jonas Pfeil, and Kay-Eberhard Gottschalk "Combining metal induced energy transfer and atomic force microscopy to probe the mechanoresponse of a focal adhesion", Proc. SPIE 11967, Single Molecule Spectroscopy and Superresolution Imaging XV, 119670A (2 March 2022); https://doi.org/10.1117/12.2608108

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