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Microscopy through inhomogeneous media distorts the wavefront of the transmitted light and results in the loss of resolution and contrast. This wavefront distortion may be compensated by the use of adaptive optics (AO). The state-of-the-art AO systems for commercial microscopes generally use deformable mirrors for wavefront modulation, which are difficult to integrate as they require the use of folded imaging paths. Herein, we present a compact and miniaturized AO ’add-on’ that fits between an objective and the turret as a simpler ’plug-and-play’ alternative. The AO add-on features a deformable phase plate - a transmissive, optofluidic wavefront modulator that is capable of wavefront correction up to 7th radial Zernike modes within a clear aperture of 10 mm, and open-loop control based-on sensorless wavefront estimation.
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Alex Dorn, Kaustubh Banerjee, Pouya Rajaeipour, Hans Zappe, Çağlar Ataman, "Compact refractive objective add-on for adaptive optics microscopy," Proc. SPIE 11969, Adaptive Optics and Wavefront Control for Biological Systems VIII, 1196903 (2 March 2022); https://doi.org/10.1117/12.2609746