Effects of CO2 and HeNe laser radiation on rat sciatic nerve in vitro

Simon Arber; Djordje Boskov; William Zev Rymer

doi:10.1117/12.17628

1 June 1990 Effects of CO₂ and HeNe laser radiation on rat sciatic nerve in vitro

Simon Arber, Djordje Boskov, William Zev Rymer

Proceedings Volume 1202, Laser-Tissue Interaction; (1990) https://doi.org/10.1117/12.17628
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Isolated rat sciatic nerve was exposed to 10.6 and 0.622 un laser radiation in a plexiglass chamber perfused with Krebs solution. A LS88OE Surgical Laser System supplied the laser beam which was aimed at the nerve laying on a zinc selenide window mounted into the chamber bottom. The nerve was Irradiated with CO2 laser CW radiation at 40, 70, 100, and 150 W/cm2 for 1, 3, 5 and 10 seconds and with pulse radiation (pulse duration 20 ms, frequency 10 Hz, 150 W/cm2) for 1 minute. The compound action potential (CAP) was recorded at the end of exposure periods and compared to that recorded right before irradiation. Two effects of the CO laser radiation on the CAP were seen to depend on the intensity and exposure time. One manifested itself in narrowing of the CAP, shifting it to the shorter latencies and a slight change in amplitude; it was completely reversible upon cessation of irradiation. The other effect was irreversible in that the reduced amplitude was not fully restored after irradiation. The irreversible conduction block had a temperature threshold around 42-45°C. The exposure to HeNe laser radiation at 0.6101 W/cm2 was conducted in the dark for 10 sec. 1, 5, 10, and 20 mm. Under no cOnditions of HeNe laser irradiation was an effect observed on the parameters of the CAP.

Citation Download Citation

Simon Arber, Djordje Boskov, and William Zev Rymer "Effects of CO₂ and HeNe laser radiation on rat sciatic nerve in vitro", Proc. SPIE 1202, Laser-Tissue Interaction, (1 June 1990); https://doi.org/10.1117/12.17628

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