Picosecond resolution study of intramolecular energy transfer in lumazine protein

John W. Lee; Gary R. Holtom; Dennis J. O'Kane

doi:10.1117/12.17750

1 May 1990 Picosecond resolution study of intramolecular energy transfer in lumazine protein

John W. Lee, Gary R. Holtom, Dennis J. O'Kane

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17750
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Lumazine protein is a 21200 Da protein containing a single tryptophan residue and a non-covalently bound, highly fluorescent ligand, 6,7-dimethyl-8-ribityllumazine. Visser et al. have reported that excitation into the region of the absorption of the tryptophan residue, around 300 nm, produces a distinct rise in fluorescence emission at 475 nm from the lumazine ligand. They analyzed a rise rate of around 1 ns and attributed this to energy transfer between the tryptophan donor and the lumazine as acceptor. This present report re-investigates this phenomenon using a ten times higher resolution (FWHM = 23 ps) . The fluorescence rise is found to be more complex and can only approximately be fitted by a sum of two exponential processes, with rise times of 0.02 and 0.6 ns. For the fluorescence of the tryptophan measured at 340 nm, no rise is detected but the decay is similarly much more complex than previously recognised with data taken at lower resolution. Global analysis of three 340 nm decay curves taken with time windows of 1.2, 4.8, and 55.6 ps/channel, results in about 5 exponential components being required for a satisfactory fit to the fluorescence decay.

Citation Download Citation

John W. Lee, Gary R. Holtom, and Dennis J. O'Kane "Picosecond resolution study of intramolecular energy transfer in lumazine protein", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17750

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available