Evaluation of microbial colony growth parameters by laser speckle imaging

Ilya Balmages; Janis Liepins; Ernests T. Auzins; Anitra Zile; Dmitrijs Bliznuks; Ilze Lihacova; Alexey Lihachev

doi:10.1117/12.2621152

27 May 2022 Evaluation of microbial colony growth parameters by laser speckle imaging

Ilya Balmages, Janis Liepins, Ernests T. Auzins, Anitra Zile, Dmitrijs Bliznuks, Ilze Lihacova, Alexey Lihachev

Proceedings Volume 12144, Biomedical Spectroscopy, Microscopy, and Imaging II; 121440B (2022) https://doi.org/10.1117/12.2621152
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Our previous studies have shown that it is possible to determine the growth activity of a microbial colony by laser speckle imaging techniques. A sub-pixel correlation method was proposed to detect small changes in the sequence of laser speckle images. In this study, we compared the laser speckle imaging method to the reference method - image time series under white light to detect the colony growth parameters (growth rate, critical detection time).

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Citation Download Citation

Ilya Balmages, Janis Liepins, Ernests T. Auzins, Anitra Zile, Dmitrijs Bliznuks, Ilze Lihacova, and Alexey Lihachev "Evaluation of microbial colony growth parameters by laser speckle imaging", Proc. SPIE 12144, Biomedical Spectroscopy, Microscopy, and Imaging II, 121440B (27 May 2022); https://doi.org/10.1117/12.2621152

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