A vectorial structured light holographic optical trap for control of fluorescent particles

Ané Kritzinger; Valeria Rodríguez-Fajardo; Sifiso A. Nsibande; Patricia B. C. Forbes; Andrew Forbes

doi:10.1117/12.2626569

24 May 2022 A vectorial structured light holographic optical trap for control of fluorescent particles

Ané Kritzinger, Valeria Rodríguez-Fajardo, Sifiso A. Nsibande, Patricia B. C. Forbes, Andrew Forbes

Proceedings Volume 12152, Mesophotonics: Physics and Systems at Mesoscale; 121520A (2022) https://doi.org/10.1117/12.2626569
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Here we create a holographic trap that allows trapping with scalar Gaussian and vortex beams, as well as vectorial combinations of the two. When equally weighted, the resultant is a propagation invariant flat-top beam trap. The set-up includes a spatial light modulator in an interferometer to combine the beams, an inverted microscope for imaging, and a photon counting fluorescence detection stage. We outline the functionality of this system, measure the trap stiffness for some example beams, and show in-situ fluorescence measurement from quantum dots attached to micro-scale beads.

Conference Presentation

Citation Download Citation

Ané Kritzinger, Valeria Rodríguez-Fajardo, Sifiso A. Nsibande, Patricia B. C. Forbes, and Andrew Forbes "A vectorial structured light holographic optical trap for control of fluorescent particles", Proc. SPIE 12152, Mesophotonics: Physics and Systems at Mesoscale, 121520A (24 May 2022); https://doi.org/10.1117/12.2626569

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