Detectability of SERS phantom in a turbid medium

Boris N. Khlebtsov

doi:10.1117/12.2624377

29 April 2022 Detectability of SERS phantom in a turbid medium

Boris N. Khlebtsov

Proceedings Volume 12194, Computational Biophysics and Nanobiophotonics; 121940V (2022) https://doi.org/10.1117/12.2624377
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

Surface-enhanced Raman scattering (SERS) tags have proven to be excellent labels for tissue bioimaging because of their low interference from biological matrices, high photostability, and possibility to use as theranostic agent. Although SERS tags are widely used for imaging of tumors in vivo, in practice, low contrast of the tags accumulation in the tissue and strong light scattering can significantly affect their detectability. In this work, we studied these effects by using a phantom of tumor tissue with incorporated SERS tags. To simulate surrounding medium with different turbidity, the phantom was placed in a 4 mm thick agarose gel containing intralipid at a concentration of 0-1%. We found that shielding the phantom with a turbid medium leads not only to a decrease in delectability but also to a decrease in the apparent size of the imaging object. For in vivo experiments we used an optical clearing technique to improve the SERS signal from a subcutaneous tumor phantom. The use of optical clearing increases the SERS signal target-to-background ratio for 5 times and allow to decrease the total imaging time for at least 10 times. In addition, SERS imaging assisted with optical clearing made it possible to more precisely determine the shape and boundaries of the implanted phantom.

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Boris N. Khlebtsov "Detectability of SERS phantom in a turbid medium", Proc. SPIE 12194, Computational Biophysics and Nanobiophotonics, 121940V (29 April 2022); https://doi.org/10.1117/12.2624377

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KEYWORDS

Raman spectroscopy

Surface enhanced Raman spectroscopy

Optical clearing

Tumors

Optical spheres

Raman scattering

Scattering

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