Presentation + Paper
14 October 2022 Quantitative phase-contrast x-ray micro CT for visualization of mouse lymph nodes
Author Affiliations +
Abstract
Previously, we designed a new quantitative phase-contrast setup for the microtomography experiments at the hereon beamlines P05 and P07 at PETRA III, DESY. This setup is based on Talbot array illuminators (TAI) as high visibility wavefront markers and can reach a spatial resolution level comparable to propagation-based imaging. In this work, we focus on the progress of bringing this setup into user operation. We collaborated with INI-Research to investigate the vascular system of mouse lymph nodes, exploiting the spatial resolution capabilities and using the quantitative aspect of the data to compare different sample preparation methods. We could successfully visualize and trace the blood supply of the lymph nodes, even with fine capillaries, showing the stability and performance of the setup in user application.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mirko Riedel, Jörg U. Hammel, Julian Moosmann, Felix Beckmann, Florian Schwarzenberg, Paul Schuetz, Stephan Henne, Clemens Wülfing, Madleen Busse, and Julia Herzen "Quantitative phase-contrast x-ray micro CT for visualization of mouse lymph nodes", Proc. SPIE 12242, Developments in X-Ray Tomography XIV, 1224213 (14 October 2022); https://doi.org/10.1117/12.2631184
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KEYWORDS
Lymphatic system

Spatial resolution

Imaging systems

Visualization

Tissues

Tomography

X-rays

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