Organoids are three-dimensional structures of self-assembled cell aggregates that mimic anatomical features of in vivo organs and, therefore, can serve as in vitro miniaturized organ models for drug testing. The most effective way of studying drug toxicity and efficacy requires fast-paced and high-resolution imaging of organoids which is possible by first immobilizing the organoids. However, suitable platforms that are conducive to high-throughput imaging and can fully immobilize the organoids are missing. We propose a unique microfluidic device capable of hydrogel-free immobilization of cardiac organoids in predetermined locations for viability staining and calcium-transients imaging without compromising their morphology and behavior. In doing so, we treated cardiac organoids with Doxorubicin (DOX), and successfully immobilized and imaged them within our device to evaluate organoid viability upon treatment.
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