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Optical-resolution photoacoustic microscopy (OR-PAM) is a label-free and non-invasive technique for imaging blood vessel and hemoglobin oxygen saturation (sO2) of living animals in vivo, providing functional information for disease diagnosis. However, most state-of-the-art OR-PAM systems require bulky and costly pulsed lasers, which hinders their wide applications in clinical settings. Here, a reflection-mode low-cost photoacoustic microscopy system using two laser diodes (LDs) was developed for in-vivo microvasculature and sO2 imaging with a high resolution of ~6 μm. The sO2 measurement is validated in both blood phantom and in vivo animal experiments. The phantom study shows that our system has a strong linear relationship with the preset sO2 (R 2 = 0.96). The in-vivo experiment of mouse ear imaging demonstrated that our system can achieve high-resolution and high-quality imaging of microvasculature and sO2. This technical advancement in cost reduction and superior imaging performance promotes the fast and wide applications of PAM in biomedical fields.
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Xiufeng Li, Bingxin Huang, Kylie Yeung, Victor T. C. Tsang, Claudia T. K. Lo, Terence T. W. Wong, "Dual-wavelength low-cost high-resolution photoacoustic microscopy system for blood oxygenation imaging," Proc. SPIE 12379, Photons Plus Ultrasound: Imaging and Sensing 2023, 1237918 (9 March 2023); https://doi.org/10.1117/12.2647870