Multi-plate continuum laser for pre-resonance coherent Raman scattering

Shi-Wei Chu

doi:10.1117/12.3008170

20 September 2023 Multi-plate continuum laser for pre-resonance coherent Raman scattering

Shi-Wei Chu

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126081R (2023) https://doi.org/10.1117/12.3008170
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Raman scattering is known to offer a label-free imaging contrast with chemical bond specificity. However, the scattering cross section of Raman interaction is extremely weak. In order to boost Raman signal strength for high-speed imaging, coherent Raman interaction and electronic pre-resonance enhancement are two potential strategies. For coherent Raman scattering, two lasers with one fixed frequency and one tunable frequency, serving as pump and Stokes beams, are necessary to match the vibrational frequencies of specific molecules or chemical bonds. On the other hand, to achieve electronic pre-resonance, it is required to tune both pump and Stokes laser frequencies simultaneously. In this work, we develop a multi-plate continuum (MPC) source that delivers octave-spanning bandwidth (600-1300 nm) and high spectral energy density (~1 nJ/cm^-1). This source not only enables coherent Raman enhancement with spectroscopic interrogation across the entire Raman active region (0-4000 cm^-1), but also provides additional two orders of magnitude enhanced signal strength through electronic pre-resonance. The imaging modalities of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) are both demonstrated with the MPC light source, on a fixed Drosophila brain tissue. We envision that utilizing MPC light source will substantially enhance the sensitivity and specificity of SRS by implementing electronic pre-resonance and spectral multiplexing via accessing three or more coherent wavelengths.

Citation Download Citation

Shi-Wei Chu "Multi-plate continuum laser for pre-resonance coherent Raman scattering", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126081R (20 September 2023); https://doi.org/10.1117/12.3008170

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
2 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman scattering

Raman spectroscopy

Laser frequency

Laser scattering

Laser bonding

Physical coherence

Vibration

Show All Keywords

Keywords/Phrases

Search In:

Publication Years