Moiré metalens-based fluorescence optical sectioning microscopy

Yu-Hsin Chia; Cheng Hung Chu; Sunil Vyas; Yi-You Huang; Din Ping Tsai; Yuan Luo

doi:10.1117/12.2676655

5 October 2023 Moiré metalens-based fluorescence optical sectioning microscopy

Yu-Hsin Chia, Cheng Hung Chu, Sunil Vyas, Yi-You Huang, Din Ping Tsai, Yuan Luo

Proceedings Volume 12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI; 126480F (2023) https://doi.org/10.1117/12.2676655
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Conference Poster

Abstract

Microscopy is an essential tool for biomedical research and clinical diagnosis. Nevertheless, conventional wide-field imaging system lacks optical sectioning ability, restricting applications in thick tissues. Recently, HiLo microscopy improved sectioning efficiency but still requires axial movement. Here, we propose the Moiré metalens to develop the varifocal metalens based fluorescence optical sectioning microscopy. It leveraging metasurfaces capabilities to control optical properties. Our system enables optical sectioning and 3D imaging capability. We demonstrate multiplane HiLo optically sectioned images of fluorescent microspheres and ex-vivo mouse brain tissues. This technique opens new possibilities for high-contrast microscopy in biomedical research and clinical diagnosis.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yu-Hsin Chia, Cheng Hung Chu, Sunil Vyas, Yi-You Huang, Din Ping Tsai, and Yuan Luo "Moiré metalens-based fluorescence optical sectioning microscopy", Proc. SPIE 12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI, 126480F (5 October 2023); https://doi.org/10.1117/12.2676655

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available