Non-invasive in vivo optogenetic pacing in mice

Marcello M. Amaral; Abigail Matt; Fei Wang; Elena Gracheva; Hongwu Liang; Attila Kovacs; Carla J. Weinheimer; Abhinav Diwan; Jianmin Cui; Stacey Rentchler; Jeanne Nerbonne; Christian Zemlin; Chao Zhou

doi:10.1117/12.3003054

13 March 2024 Non-invasive in vivo optogenetic pacing in mice

Marcello M. Amaral, Abigail Matt, Fei Wang, Elena Gracheva, Hongwu Liang, Attila Kovacs, Carla J. Weinheimer, Abhinav Diwan, Jianmin Cui, Stacey Rentchler, Jeanne Nerbonne, Christian Zemlin, Chao Zhou

Author Affiliations +

Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 128190K (2024) https://doi.org/10.1117/12.3003054
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We develop and characterize transgenic mouse models expressing ReaChR or NpHR specifically expressed in the heart tissue. Non-invasive pacing is achieved by shining red light from an LED array over a 10 mm diameter spot on the shaved mouse chest skin. We demonstrated that low power density (~1.2 mW/mm2) illumination is sufficient to induce tachycardia, bradycardia, and sustained arrhythmia, providing full control over the heart rhythm in live, anesthetized mice. This in vivo optogenetic pacing platform opens opportunities for future non-invasive studies on mammalian heart physiology, diseases, and therapies for arrhythmias without any surgical intervention.

Conference Presentation

Citation Download Citation

Marcello M. Amaral, Abigail Matt, Fei Wang, Elena Gracheva, Hongwu Liang, Attila Kovacs, Carla J. Weinheimer, Abhinav Diwan, Jianmin Cui, Stacey Rentchler, Jeanne Nerbonne, Christian Zemlin, and Chao Zhou "Non-invasive in vivo optogenetic pacing in mice", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 128190K (13 March 2024); https://doi.org/10.1117/12.3003054

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