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12 March 2024 Analysis of the spread of bacterial resistance after photodynamic inactivation by minimum inhibitory concentration (MIC)
R. V. De Lima, J. M. Soares, K. C. Blanco, V. S. Bagnato
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Proceedings Volume 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; 128220F (2024) https://doi.org/10.1117/12.3004763
Event: SPIE BiOS, 2024, San Francisco, California, United States
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Abstract
The objective of this study was to prepare mixed cultures of both sensitive and resistant bacteria of the same species in varying proportions (20% and 80%, 50% and 50% and 80% and 20%) We aimed to analyze the minimum inhibitory concentration (MIC) both before and after photoinactivation treatment. This treatment involved the use of the synthetic photosensitizer curcumin 5 mM (PDTPharma®) and irradiation with an LED lighting device (Biotable® - produced by MM Optics-Brasil) emitting a wavelength of 450 nm, at 40 mW/cm², and a light dose of 10 J/cm². The purpose was to examine the impact of photodynamic action on the spread of bacterial resistance. Combating the proliferation of antimicrobial resistance is a critical global health issue, necessitating treatment methods that adequately address this challenge.
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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R. V. De Lima, J. M. Soares, K. C. Blanco, and V. S. Bagnato "Analysis of the spread of bacterial resistance after photodynamic inactivation by minimum inhibitory concentration (MIC)", Proc. SPIE 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, 128220F (12 March 2024); https://doi.org/10.1117/12.3004763
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KEYWORDS
Resistance
Bacteria
Oxygen
LED lighting
Microorganisms
Microbiology
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