System for simultaneous measurement of fluorescence and hemodynamics (SSMFH) from deep brain regions of freely moving rodents

Anupam Bisht; Govind Peringod; Grant R. Gordon; Jaideep S. Bains; Kartikeya Murari

doi:10.1117/12.3002776

12 March 2024 System for simultaneous measurement of fluorescence and hemodynamics (SSMFH) from deep brain regions of freely moving rodents

Anupam Bisht, Govind Peringod, Grant R. Gordon, Jaideep S. Bains, Kartikeya Murari

Author Affiliations +

Proceedings Volume 12828, Neural Imaging and Sensing 2024; 128280A (2024) https://doi.org/10.1117/12.3002776
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Our understanding of hemodynamic signals, blood perfusion and oxygen saturation (sO₂), recorded through fiber-based instruments is limited. To address this, a potential method can be to simultaneously acquire hemodynamic signals with widely used fluorescence fiber photometry signals. We report a novel System for the Simultaneous Measurement of Fluorescence and Hemodynamics (SSMFH) from deep brain regions of freely moving rodents. SSMFH has been developed by modifying our previous white light reflectance single fiber system (SFS) that enabled measurement of blood perfusion and oxygen saturation from freely moving rodents. SSMFH has been designed to be easily integrated with a commercial fluorescence fiber photometry system for time-locked measurement of both hemodynamics and fluorescence signals. SSMFH can be additionally synchronized to behavioral monitoring cameras and other behavioral equipment during experiments. In contrast to previous work in the literature, SSMFH enables hemodynamic recordings from deep brain regions using a wide spectral range (∼ 545nm to 700nm) without the need for injection of an activity-independent fluorescent reporter. The details of the design will be presented along with data to illustrate proof-of-concept through an animal experiment. GCaMP-based fiber photometry and its relation to neuronal activity is well understood. Hemodynamic measurement with simultaneous and co-localized GCaMP based neuronal activity recording can help in understanding variations in in-vivo hemodynamic signals. SSMFH can be used to correct fluorescence measurements which are affected by blood absorption changes.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Anupam Bisht, Govind Peringod, Grant R. Gordon, Jaideep S. Bains, and Kartikeya Murari "System for simultaneous measurement of fluorescence and hemodynamics (SSMFH) from deep brain regions of freely moving rodents", Proc. SPIE 12828, Neural Imaging and Sensing 2024, 128280A (12 March 2024); https://doi.org/10.1117/12.3002776

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