Integrated opto-fluidic device for high-power living cell exposure and detection

Arnoud S. Everhardt; Vasileios Konstantinidis; Paul Kapteijn; Marcel Hoekman; Soultana Konstantinidou; Marta D'Amora; Alessandro De Carli; Francesco Fuso; Francesco Tantussi; Michele Lai; Vittoria Raffa; Sadoon Al-Obaidi; Noor Schilder; René Heideman; Douwe Geuzebroek; Frederik Schreuder

doi:10.1117/12.3003176

12 March 2024 Integrated opto-fluidic device for high-power living cell exposure and detection

Arnoud S. Everhardt, Vasileios Konstantinidis, Paul Kapteijn, Marcel Hoekman, Soultana Konstantinidou, Marta D'Amora, Alessandro De Carli, Francesco Fuso, Francesco Tantussi, Michele Lai, Vittoria Raffa, Sadoon Al-Obaidi, Noor Schilder, René Heideman, Douwe Geuzebroek, Frederik Schreuder

Author Affiliations +

Proceedings Volume 12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; 128370C (2024) https://doi.org/10.1117/12.3003176
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Genome editing comprises the most promising work in 21st century genetics, with molecular surgery as its practical horizon. For a successful clinical application of molecular surgery, e.g. to treat cancer, safe and efficient editing is crucial. Light-induced molecular surgery is the perfect tool for its excellent control over wavelength, power, and exposure time. Light is also an excellent tool for the detection of (living) cells. These modalities are ideal for opto-fluidics: combining integrated photonics and microfluidics in a chip. In this work, we used the TriPleX^® waveguide platform, comprised of silicon nitride and silicon oxide, to control high power (>1W) visible light. Living cells are inserted into a 100 micrometer wide microfluidic channel after which they are focused into a 25 micrometer wide section in its center using side-, backand lift sheath flows. The cells can be readily recovered at the microfluidic channel’s output with >90% survival rate. Chemically deactivated CRISPR/Cas9 molecules are activated by the laser light for safe molecular surgery. In parallel, it is possible to detect living cells flowing in the microfluidic channel. Measuring light absorption by the analyte makes it possible to detect each individual cell passing by the laser light, and to microscopically verify that >97% of the cells are correctly centered in the microfluidic channel. This device represents a first step to a fully integrated on-chip flow cytometer. Early results demonstrate its efficacy in cell detection and controllable exposure, paving the way to safe molecular surgery.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Arnoud S. Everhardt, Vasileios Konstantinidis, Paul Kapteijn, Marcel Hoekman, Soultana Konstantinidou, Marta D'Amora, Alessandro De Carli, Francesco Fuso, Francesco Tantussi, Michele Lai, Vittoria Raffa, Sadoon Al-Obaidi, Noor Schilder, René Heideman, Douwe Geuzebroek, and Frederik Schreuder "Integrated opto-fluidic device for high-power living cell exposure and detection", Proc. SPIE 12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, 128370C (12 March 2024); https://doi.org/10.1117/12.3003176

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