Plasmonic biosensing is an area of research where scientists are developing novel devices and concepts. The validation of these devices requires thorough testing of key characteristics, including accuracy, specificity, robustness, and linearity, all of which are interconnected with a system's limit of detection. By optimizing parameters to meet these criteria, the development of highly sensitive and specific biosensing devices can be facilitated, allowing for effective performance comparisons with other biosensing devices. The limit of detection is a measure of the ability to detect small traces of molecules in each solution, and it can be used to measure the performance of a biosensing system. In this study, we evaluated the limit of detection of a home-built localized surface plasmon resonance (LSPR) system. To this end, gold-coated and titanium-coated slides were exposed to neutravidin and biotinylated probes at varying concentrations ranging from 100 μM to 6.125 μM, without the presence of an analyte. By analyzing the transmitted intensity against concentration, we constructed a reliable sensing curve. Through linear regression analysis, we determined the slope of the linear calibration function with high precision. By evaluating the limit of detection, this study will contribute to the understanding of the performance capabilities of the localized surface plasmon resonance technique. The findings presented here serve as a valuable benchmark for developing and comparing highly sensitive and specific biosensing devices.
|