Assessment of the biomechanical changes on zebrafish larvae eye development caused by phenylthiourea (PTU) treatment using reverberant optical coherence elastography (Rev-OCE)

Christian Zevallos-Delgado; Taye T. Mekonnen; Yogeshwari S. Ambekar; Manmohan Singh; Joshua D. Wythe; Salavat R. Aglyamov; Kirill V. Larin

doi:10.1117/12.3003390

12 March 2024 Assessment of the biomechanical changes on zebrafish larvae eye development caused by phenylthiourea (PTU) treatment using reverberant optical coherence elastography (Rev-OCE)

Christian Zevallos-Delgado, Taye T. Mekonnen, Yogeshwari S. Ambekar, Manmohan Singh, Joshua D. Wythe, Salavat R. Aglyamov, Kirill V. Larin

Author Affiliations +

Proceedings Volume 12844, Optical Elastography and Tissue Biomechanics XI; 1284406 (2024) https://doi.org/10.1117/12.3003390
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Phenylthiourea (PTU) is often used to block pigmentation and make zebrafish completely transparent for easy optical imaging. PTU inhibits melanogenesis by inhibiting tyrosinase. Although the PTU is commonly used, it does have some side effects. PTU at a concentration of 0.2 mM (0.003%) significantly reduces the zebrafish eye size due to the inhibition of thyroid hormone production. Furthermore, low levels of thyroid hormones in zebrafish increase the stiffness of the intervertebral joints, altering their swimming behavior. The aim of this study was to assess the structural modifications and biomechanical properties of 5-day post-fertilization (dpf) zebrafish eyes after being exposed to PTU using optical coherence tomography and reverberant optical coherence elastography, respectively. Wild-type zebrafish (n=3), treated with PTU (0.2 mM), were compared with non-treated zebrafish (n=3). The results show a significant reduction (p=0.02) in the mean eye diameter of the fishes treated with PTU (312.66 ± 8.71 μm) versus the non-treated group (340.18 ± 4.38 μm). On the other hand, the non-treated group showed a significantly slower (p=0.02) shear wave speed (0.97 ± 0.12 m/s) compared with the PTU-treated group (2.65 ± 0.51 m/s), indicating that PTU induces a biomechanical change in the stiffness of the developing eye. PTU is a potent inhibitor of the pigmentation of zebrafish; however, it can also severely affect its biomechanical properties, specifically eye development, reducing eye diameter and increasing its stiffness.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Christian Zevallos-Delgado, Taye T. Mekonnen, Yogeshwari S. Ambekar, Manmohan Singh, Joshua D. Wythe, Salavat R. Aglyamov, and Kirill V. Larin "Assessment of the biomechanical changes on zebrafish larvae eye development caused by phenylthiourea (PTU) treatment using reverberant optical coherence elastography (Rev-OCE)", Proc. SPIE 12844, Optical Elastography and Tissue Biomechanics XI, 1284406 (12 March 2024); https://doi.org/10.1117/12.3003390

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