Quantitative multiplexed SWIR imaging to study nanomedicine surface coatings in vivo

Xingjian Zhong; Amish Patel; Yidan Sun; Mallory A. Moffett; Allison M. Dennis

doi:10.1117/12.3003317

13 March 2024 Quantitative multiplexed SWIR imaging to study nanomedicine surface coatings in vivo

Xingjian Zhong, Amish Patel, Yidan Sun, Mallory A. Moffett, Allison M. Dennis

Proceedings Volume 12859, Colloidal Nanoparticles for Biomedical Applications XIX; 1285902 (2024) https://doi.org/10.1117/12.3003317
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Shortwave infrared (SWIR) imaging capitalizes on the low tissue scattering and low autofluorescence in the near infrared- II (NIR-II) window (1000 nm – 1700 nm) for in vivo imaging with deep imaging penetration and high signal-to-noise contrast. Combining NIR-I excitation and emission in SWIR window, quantitative multiplexed imaging could be applied to study biological structures or physiological phenomenon in a single specimen. This study introduces a set of three high quantum yield lead sulfide/cadmium sulfide (PbS/CdS) core/shell quantum dots (QDs) with distinct SWIR emissions (1100 – 1550 nm). Applying these QDs, we demonstrated detailed lymphatic pathway, lymphatic drainage, and spatially overlapping vascular structures, marking a significant advancement beyond the conventional two-color schemes in SWIR imaging. We further evaluated the effect of surface coatings of the QDs on the pharmacokinetics and biodistribution of QDs in mice. The capacity to differentiate several fluorescent contrast agents through SWIR detection unlocks numerous opportunities for studies of disease progression, drug pharmacokinetics and biodistribution, and cell trafficking dynamics in living organisms.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xingjian Zhong, Amish Patel, Yidan Sun, Mallory A. Moffett, and Allison M. Dennis "Quantitative multiplexed SWIR imaging to study nanomedicine surface coatings in vivo", Proc. SPIE 12859, Colloidal Nanoparticles for Biomedical Applications XIX, 1285902 (13 March 2024); https://doi.org/10.1117/12.3003317

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