Common-path off-axis differential interference contrast microscope for monitoring cell dynamics

Anuj Saxena; Azeem Ahmad; Vishesh Dubey; Hong Mao; Anand Kumar; Anowarul Habib; Satish Kumar Dubey; Balpreet Singh Ahluwalia; Dalip Singh Mehta

doi:10.1117/12.3017023

18 June 2024 Common-path off-axis differential interference contrast microscope for monitoring cell dynamics

Anuj Saxena, Azeem Ahmad, Vishesh Dubey, Hong Mao, Anand Kumar, Anowarul Habib, Satish Kumar Dubey, Balpreet Singh Ahluwalia, Dalip Singh Mehta

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Proceedings Volume 12996, Unconventional Optical Imaging IV; 129960T (2024) https://doi.org/10.1117/12.3017023
Event: SPIE Photonics Europe, 2024, Strasbourg, France

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Abstract

Differential Interference Contrast (DIC) microscopy is a label-free technique crucial for visualizing transparent biological specimens. In this study, we introduce a novel DIC interferometry imaging technique based on calcite beam displacer (CBD), significantly enhancing resolution and contrast. Our system, requires only two polarizers and a calcite beam displacer, offers a practical and straightforward implementation on standard microscopes. We demonstrate its efficacy by imaging human red blood cells (RBCs) and bacteria, retrieving differential phase information, and recording the dynamics of U2OS cells over an hour at 15 frames per second (fps). This advancement in DIC microscopy holds promise for broader applications in high-resolution imaging of transparent specimens in cell biology research.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Anuj Saxena, Azeem Ahmad, Vishesh Dubey, Hong Mao, Anand Kumar, Anowarul Habib, Satish Kumar Dubey, Balpreet Singh Ahluwalia, and Dalip Singh Mehta "Common-path off-axis differential interference contrast microscope for monitoring cell dynamics", Proc. SPIE 12996, Unconventional Optical Imaging IV, 129960T (18 June 2024); https://doi.org/10.1117/12.3017023

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