Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT

Torben Hildebrand; Qianli Ma; Catherine Anne Heyward; Håvard J. Haugen; Liebert P. Nogueira

doi:10.1117/12.3027063

4 October 2024 Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT

Torben Hildebrand, Qianli Ma, Catherine Anne Heyward, Håvard J. Haugen, Liebert P. Nogueira

Proceedings Volume 13152, Developments in X-Ray Tomography XV; 131520J (2024) https://doi.org/10.1117/12.3027063
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Abstract

Micro-CT, widely used for analyzing hard tissues like bone, faces challenges when inspecting soft tissues due to low contrast. Here, we introduce a method to enhance the contrast between adjacent soft and hard tissues by reducing hard tissue attenuation through decalcification and by increasing soft tissue attenuation through contrast enhancement. By exploring contrast-enhanced micro-CT utilizing Lugol’s iodine, iodine in ethanol, and phosphotungstic acid (PTA) for cellular components in bone on 28-day-old B6 mice femur samples, Lugol’s iodine displayed high specificity to cellular structures. This resulted in outstanding contrasts of cellular components due to low binding to prior decalcified bone or cartilage. This approach, employing both phase-contrast and absorption micro-CT techniques, holds promise for detailed investigations into the cellular details of bone tissues, offering potential advancements in its understanding of bone and its soft tissue components.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Torben Hildebrand, Qianli Ma, Catherine Anne Heyward, Håvard J. Haugen, and Liebert P. Nogueira "Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT", Proc. SPIE 13152, Developments in X-Ray Tomography XV, 131520J (4 October 2024); https://doi.org/10.1117/12.3027063

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