Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy

Hongli Ni; Yuhao Yuan; Mingsheng Li; Yifan Zhu; Xiaowei Ge; Jiaze Yin; Chinmayee Prabhu Dessa; Le Wang; Ji-Xin Cheng

doi:10.1117/12.3034752

21 October 2024 Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy

Hongli Ni, Yuhao Yuan, Mingsheng Li, Yifan Zhu, Xiaowei Ge, Jiaze Yin, Chinmayee Prabhu Dessa, Le Wang, Ji-Xin Cheng

Proceedings Volume 13186, SPIE Translational Biophotonics + Additive Manufacturing for Photonics 2024; 131861H (2024) https://doi.org/10.1117/12.3034752
Event: SPIE Translational Biophotonics + Additive Manufacturing for Photonics, 2024, Houston, Texas, United States

Conference Poster

Abstract

Deep-tissue chemical imaging is essential for many biomedical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with micron lateral resolution. By pumping the overtone transition of carbon-hydrogen bonds and probing the subsequent photothermal lens with shortwave infrared light, SWIP can obtain chemical contrast from one-micron polymer particles located at 800-μm depth in a highly scattering phantom. We demonstrated that SWIP can resolve intracellular lipids across an intact tumor spheroid and the layered structure in thick liver, skin, brain, and breast tissues. SWIP microscopy fills a gap in vibrational imaging with sub-cellular resolution and millimeter-level penetration, which heralds broad potential for life science and clinical applications.

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Hongli Ni, Yuhao Yuan, Mingsheng Li, Yifan Zhu, Xiaowei Ge, Jiaze Yin, Chinmayee Prabhu Dessa, Le Wang, and Ji-Xin Cheng "Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy", Proc. SPIE 13186, SPIE Translational Biophotonics + Additive Manufacturing for Photonics 2024, 131861H (21 October 2024); https://doi.org/10.1117/12.3034752

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