With the progress of molecular diagnostic technologies, manual nucleic acid extraction methods no longer suffice to meet current demands. In order to enhance the positioning precision and liquid handling accuracy of a 25-well high-throughput automated pipetting workstation, we investigated an S-shaped acceleration-deceleration algorithm based on the Sigmoid function for controlling stepper motors along the X, Y, and Z axes. Subsequently, we constructed a corresponding mechanical device. Through this apparatus, we conducted experiments to assess positioning precision, liquid handling accuracy, and biological performance. The experimental results revealed that the absolute error in positioning precision was less than 0.1 mm, and the coefficient of variation (CV) for liquid handling accuracy was below 1%. Furthermore, post-purification nucleic acid A260/A280 ratios were measured at approximately 1.911, falling within the range of 1.8 to 2.0. Our devised system further improved both positioning and liquid handling precision, thereby advancing high-throughput automated nucleic acid extraction technology.
|